NEWS
Unlock the power of live-cell kinetic monitoring for your GPCR research with Promega GloSensor™ technology.
Understanding GPCR signaling in real time has long been a challenge for researchers. Traditional cAMP assay methods often require cell lysis, provide only endpoint data, or lack the sensitivity needed for kinetic studies. As the authorized distributor of Promega in Hong Kong, we offer the GloSensor™ technology to change that— providing a bioluminescent approach to monitor intracellular cAMP dynamics in living cells with exceptional precision.
How the GloSensor™ cAMP Assay Works
The GloSensor™ platform is built around an engineered firefly luciferase that serves as a reversible cAMP biosensor. When cAMP levels rise upon GPCR activation, the biosensor undergoes a conformational change, generating a luminescent signal that can be tracked continuously.
- Transfect cells with a pGloSensor™ cAMP plasmid
- Stimulate cells with test compounds or reference ligands
- Incubate with GloSensor™ Reagent and detect real-time luminescence
Why Choose GloSensor™ for your Laboratory?
Live-Cell Kinetic Monitoring
Unlike endpoint assays, GloSensor™ technology allows you to follow signal transduction over time—capturing rapid activation, desensitization, and recovery phases in the same sample.
No Cell Lysis Required
Cells remain intact throughout the experiment. This preserves the physiological context and allows for downstream analyses on the same cell population, a major advantage for cAMP assay HK labs focusing on precious samples.
High Sensitivity & Wide Dynamic Range
Engineered from native luciferase, GloSensor™ cAMP Assay delivers robust signal-to-noise performance suitable for high-throughput screening in 384- and 1536-well formats.
Versatile Platform Compatibility
The assay is compatible with standard multimode plate readers equipped with luminescence detection, making it accessible for most laboratories. There is no need for specialized imaging equipment to get high-quality data.
Applications
GloSensor™ technology supports a broad range of GPCR-related research and PDE assay applications:
• Gs- and Gi-coupled GPCR screening – Distinguish between activating and inhibitory pathways in a single assay
• Real-time cAMP dose-response studies – Generate full kinetic profiles for agonist and antagonist characterization
• GPCR desensitization and internalization – Track receptor behavior beyond initial activation
• cAMP pathway analysis in disease models – Investigate signaling dysregulation in relevant cell types
• PDE inhibitor screening – Measure functional effects on cAMP turnover with high temporal resolution using our PDE assay protocols.
• Inverse agonist detection – Identify compounds that reduce constitutive receptor activity
For researchers seeking to bridge the gap between molecular signaling and cellular physiology, GloSensor™ technology offers a reliable, live-cell solution. By providing continuous readouts of cAMP dynamics, it enables deeper characterization of GPCR pharmacology, from early-stage target validation to mechanistic studies.
Whether working with endogenous receptors or engineered cell lines, the GloSensor™ platform delivers data that more closely reflects the complexity of biological signaling.
For more information on GloSensor™ technology, including detailed protocols and technical assistance, please contact us!
【技術亮點】即時掌握cAMP與GPCR訊號：GloSensor™ 活細胞偵測技術
透過 Promega GloSensor™ 技術，解鎖活細胞動力學監測的強大功能，提升您的 GPCR 研究效率。
在生命科學研究中，即時觀察活細胞中的GPCR活性，過去常受限於傳統的 cAMP 檢測 (cAMP assay)方法，需要破碎細胞，僅能取得終點數據，或靈敏度不足。Promega GloSensor™ 技術以生物冷光為基礎，讓研究人員能在活細胞中連續追蹤cAMP的動態變化，提供更貼近生理狀態的實驗視角。
GloSensor™技術原理與流程
GloSensor™ 利用改造後的螢光酵素作為cAMP生物感應器。當GPCR被活化、細胞內cAMP濃度上升時，感應器會改變構型並產生冷光訊號，訊號強度與cAMP濃度成正比。
• 轉染 pGloSensor™ cAMP 質體
• 加入測試化合物刺激細胞、配體或進行 PDE 檢測相關實驗。
• 加入 GloSensor™ 試劑，即時偵測冷光訊號
為何選擇 GloSensor™？
活細胞即時偵測
GloSensor™ 技術讓你能持續追蹤訊號活化的動態變化，包括快速反應、去敏感化與恢復階段，是 香港 GPCR 檢測 研究的首選工具。
無需裂解細胞，保留生理完整性
細胞在實驗過程中保持完整，不僅保留了最真實的生理背景，還能支持後續的其他分析實驗，極大化實驗樣本的利用率。
高靈敏度與寬廣動態範圍
適用於高通量篩選平台，穩定支援384孔與1536孔盤格式。
實驗流程簡便
操作流程與一般冷光偵測相容，無需特殊儀器即可導入現有實驗系統。
應用領域
• Gs與Gi耦合GPCR篩選
• 即時cAMP劑量反應分析
• GPCR去敏感化與內化研究
• 疾病模型中的cAMP路徑分析
• PDE抑制劑篩選以優化 PDE 檢測實驗流程
• 逆性活化劑偵測
GloSensor™ 技術讓研究人員能以更接近生理條件的方式，解析GPCR的動態行為。無論是內源性受體或過表現細胞株，都能提供穩定、即時且具備生物意義的cAMP訊號數據，協助您更深入了解受體藥理特性。
如需更多關於 GloSensor™ 技術的資訊，包含詳細操作流程與技術支援，歡迎與我們聯絡！
