NEWS
Exosome isolation — specifically, isolating high-quality extracellular vesicles (EVs) — has always forced researchers to choose between yield and purity. System Biosciences (SBI) has eliminated this trade-off with the ExoQuick® UltraPure EV isolation kit. As the authorized SBI distributor in Hong Kong, we are proud to offer this next-generation hybrid solution that combines gentle precipitation with advanced spin column technology. It delivers maximum yields of pure, apoB-free, intact EVs in just 20 minutes—serving as the perfect ultracentrifuge alternative.
How the ExoQuick® UltraPure EV Isolation Kit Works
ExoQuick® UltraPure uses a dual action mechanism for superior extracellular vesicle enrichment: first, a proprietary precipitation buffer enriches EVs from small sample volumes; then, mixed mode spin columns remove high abundance contaminants like albumin and ensure efficient apoB removal, while intact EVs flow through for immediate downstream use.
- Step 1: Add ExoQuick to 100–250 µL serum/plasma, incubate at 4°C (30 min or overnight).
- Step 2: Spin at 12,000×g for 10 minutes to collect EV‑enriched pellet.
- Step 3: Resuspend EVs and add to pre‑washed ExoQuick UltraPure purification column.
- Step 4: Spin at 1,000×g for 1 minute – pure EVs collected in flow‑through.
Why Choose ExoQuick® UltraPure?
Superior Yield, Superior Purity
Unlike ultracentrifugation (UC) or size exclusion chromatography (SEC), ExoQuick® UltraPure delivers both high recovery and clean isolates:
- Up to 3,500× higher yield than UC
- >25× higher yield than SEC
- ~96% ApoB removal and reduced albumin carryover
- Strong retention of EV markers (CD9, CD81, TSG101)
- BioAnalyzer verified RNA quality.
Small Sample, Fast Workflow
Start with just 100–250 µL of serum or plasma. Designed for researchers needing high-quality serum EV purification or plasma exosome kit solutions, it delivers pure EVs from sample in just 20 minutes—no ultracentrifuge, no overnight runs, no specialized equipment.
Broad Applications
ExoQuick® UltraPure supports a wide range of EV research:
- EV nucleic acid & protein profiling
- EV quantification & characterization
- EV engineering & cargo loading
- In vivo / ex vivo EV delivery
- Biomarker discovery
- Therapeutic development
Ideal for researchers who need pure, intact EVs with high recovery, especially from limited samples. Have questions? Contact us!
System Biosciences ExoQuick® UltraPure 外泌體分離試劑組 — 高產量、高純度，無需超速離心
分離高品質胞外囊泡（EVs）時，研究人員常在「產量」與「純度」之間取捨。System Biosciences (SBI) 的 ExoQuick® UltraPure外泌體分離試劑組徹底解決這個難題。作為 System Biosciences 香港的授權供應商，我們推薦此款次世代混合型 EV 分離試劑組，結合溫和沉澱法與先進離心管柱技術，僅需 20 分鐘即可獲得最高產量的純淨、無 apoB、完整 EVs——無需超速離心機。
技術原理
ExoQuick® UltraPure 採用雙重作用機制，實現高效的胞外囊泡分離：首先以專利沉澱試劑從微量樣本中富集 EVs，再透過混合模式離心管柱去除 apoB 和白蛋白等高豐度污染物，同時完整 EVs 會直接流過管柱，可立即用於下游實驗。
- 步驟1: 於 100–250 µL 血清/血漿中加入ExoQuick，4°C 孵育 30 分鐘或過夜
- 步驟 2: 2,000×g 離心 10 分鐘，收集富含 EV 的沉澱物
- 步驟 3: 懸浮 EVs，加入預洗過的 ExoQuick UltraPure 純化管柱
- 步驟 4: 1,000×g 離心 1 分鐘 – 純化 EVs 收集於流穿液中
為何選擇ExoQuick® UltraPure?
更高產量、更高純度
相較於超速離心法（UC）或尺寸排阻層析法（SEC），ExoQuick® UltraPure 能同時實現高回收率與乾淨的樣本：
• 產量比 UC 高 3,500 倍
• 產量比 SEC 高 25 倍
• 去除約 96% 的 ApoB，並減少白蛋白殘留
• 良好保留 EV 標誌蛋白（CD9、CD81、TSG101）
• 經 BioAnalyzer 驗證的 RNA 品質
微量樣本，快速流程
無論您是在尋找 血漿外泌體解決方案還是 EV 純化試劑盒，ExoQuick® UltraPure 都能滿足您的需求。無需超速離心機，無需過夜操作，無需特殊設備，僅需 100–250 µL 血清或血漿，從樣本到純化 EVs 只需 20 分鐘。
廣泛應用
ExoQuick® UltraPure 可支援多種 EV 研究領域：
• EV 核酸與蛋白質分析
• EV 定量與特性鑑定
• EV 工程改造與貨物裝載
• 體內／離體 EV 遞送
• 生物標誌物發現
• 治療開發
對於需要高純度、完整 EVs 且追求高回收率（尤其是樣本量有限時）的研究人員，ExoQuick® UltraPure 提供了簡單、快速且可靠的替代方案，無需使用 UC 或 SEC。如需更多資訊，歡迎與我們聯絡！
