Achieving high transfection efficiency while maintaining optimal cell viability is a constant challenge for researchers, especially when working with sensitive or hard-to-transfect cell types. Traditional reagents often compromise cell health or require complex optimization steps. 

As the authorized distributor of Promega in Hong Kong, we offer the ViaFect™ Transfection Reagent, a superior solution for DNA delivery. Designed specifically for hard-to-transfect cells, ViaFect™ minimizes cytotoxicity, making it ideal for primary cell transfection, stem cell transfection, and immune cell transfection.

How It Works
ViaFect™ is a proprietary, lipid-based formulation designed to form stable complexes with DNA. These complexes are efficiently taken up by cells, enabling robust transgene expression while preserving cell health.

• Flexible Preparation: Prepare DNA-ViaFect™ complexes in serum-free or serum-containing media.
• Streamlined Workflow: Add complexes directly to cells—no media change required.
• Reliable Analysis: Proceed directly to gene expression or downstream functional assays.

Gentle on Cells, High in Efficiency
Unlike many transfection reagents that can induce significant cytotoxicity, ViaFect™ maintains high cell viability even in sensitive cell types. This low cytotoxicity approach ensures your experimental results reflect true biological outcomes, not stress-induced artifacts.

Simple Protocol, Minimal Optimization
ViaFect™ is compatible with both adherent and suspension cell cultures and works effectively in the presence of serum and antibiotics. Its straightforward protocol eliminates the need for media changes before or after transfection, streamlining your workflow and reducing hands-on time.

Broad Cell Type Compatibility
ViaFect™ delivers consistent performance across a wide range of cell lines:

• Primary cells (e.g., neurons, hepatocytes)
• Stem cells
• Immune cells (e.g., T cells, macrophages)
• Traditionally hard-to-transfect lines (e.g., Jurkat, K562, THP-1)

Versatile Applications
Whether you are performing CRISPR transfection or large-scale production, ViaFect™ supports diverse research needs:

• CRISPR/Cas9 genome editing – Efficient delivery of Cas9 and guide RNA constructs
• Viral vector production – High-yield packaging for lentivirus, AAV, and retrovirus
• Stable cell line generation – Maintain viability through selection and clonal expansion
• Gene expression studies – Reliable transient transfection for overexpression or knockdown
• Primary cell research – Achieve meaningful transfection efficiency where other reagents fall short

Why Choose Promega ViaFect™ Transfection Reagent?
For researchers working with challenging cell types or seeking a gentler alternative to conventional transfection reagents, ViaFect™ offers a trusted, easy-to-use solution. Its combination of high efficiency, low cytotoxicity, and protocol simplicity enables more reproducible results across your most demanding experimental systems.

Whether you are establishing CRISPR-edited cell lines, producing viral vectors, or studying gene function in primary cells, ViaFect™ delivers the performance you need—without compromising cell health.

Ready to improve your transfection results? Contact us today for detailed protocols and technical assistance or to order ViaFect™!

【技術亮點】Promega ViaFect™ 轉染試劑：溫和高效，專為難轉染細胞設計
在進行基因傳遞實驗時，如何在維持高轉染效率的同時兼顧細胞健康，一直是研究人員面臨的挑戰，尤其是使用初代細胞、幹細胞或免疫細胞等敏感細胞株時。Promega 的 ViaFect™ 轉染試劑提供了可靠的解決方案，能以低細胞毒性實現高效的 DNA 傳遞，特別適用於難轉染細胞模型。

技術原理
ViaFect™ 採用專利脂質配方，能與 DNA 形成穩定的複合體。這些複合體可被細胞高效攝入，在維持細胞健康的同時，實現穩定的外源基因表現。

• 靈活製備：於含血清或不含血清的培養基中製備 DNA-ViaFect™ 複合體
• 簡化流程：直接將複合體加入細胞，無需更換培養基
• 高效分析：快速進行基因表現分析或下游功能性實驗

溫和高效，兼顧細胞健康
與許多會造成顯著細胞毒性的轉染試劑不同，ViaFect™ 即使在敏感細胞株中也能維持高細胞存活率。這種溫和的特性確保實驗結果反映真實的生物學反應，而非細胞受迫所產生的偽影。

操作簡單，優化門檻低
ViaFect™ 同時適用於貼附型與懸浮型細胞，並可在含血清及抗生素的培養基中使用。簡便的操作流程無需在轉染前後更換培養基，能有效簡化實驗步驟、縮短操作時間。

廣泛的細胞株相容性
ViaFect™ 在多種細胞株中均能提供穩定的細胞轉染表現，包括：

• 初代細胞（如神經元、肝細胞）
• 幹細胞：專為幹細胞轉染優化
• 免疫細胞（如 T 細胞、巨噬細胞）適用於免疫細胞轉染
• 傳統上難以轉染的細胞株（如 Jurkat、K562、THP-1）

多元應用領域
無論你是正在進行CRISPR轉染還是大規模生產，ViaFect™可支援多種基因傳遞相關研究：

• CRISPR/Cas9 基因編輯 – 高效傳遞 Cas9 與引導 RNA 構築體
• 病毒載體生產 – 用於慢病毒、AAV 及反轉錄病毒的高產量包裝
• 穩定細胞株建立 – 在篩選與單株擴增過程中維持細胞健康
• 基因表現研究 – 可靠的暫時性轉染，適用於過度表現或基因靜默實驗
• 初代細胞研究 – 在其他試劑效果有限的情況下，仍可達成具意義的轉染效率

為什麼選擇 ViaFect™？
對於使用難轉染細胞株或尋求溫和轉染方案的研究人員而言，ViaFect™ 提供了一個值得信賴且操作簡便的選擇。結合高效轉染、低細胞毒性與簡易的操作流程，能協助你在廣泛的實驗系統中獲得更加穩定的實驗結果。
無論是建立 CRISPR 基因編輯細胞株、生產病毒載體，或是在初代細胞中研究基因功能，ViaFect™ 都能在確保細胞健康的同時，提供你所需的轉染表現。

想要提升您的轉染效果嗎？立即聯絡我們，取得詳細的操作流程和技術支援，或訂購 ViaFect™轉染試劑！

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