NEWS
3D cell culture (spheroid culture and organoid culture) is now standard in drug discovery, cancer research, and tissue engineering. But extracting high-quality total RNA (including miRNA) from microspheres or Corning Matrigel® matrix used to be challenging – until now.
Promega’s ReliaPrep™ miRNA Miniprep System, combined with Corning® Ultra-Low Attachment (ULA) plates or Matrigel® matrix, delivers a complete, validated workflow for superior RNA extraction and RNA isolation from 3D cultures.
(Note: Reliaprep RNA Cell Miniprep and Reliaprep RNA Tissue Miniprep systems are consolidating into a single unified ReliaPrep™ RNA Miniprep System.)
Proven Protocol Highlights
1.Grow cells in Corning ULA plates or Matrigel® matrix
2.Gently homogenize with LBA + TG buffer
3.Follow the simple spin-column protocol (full details in TM469)
4.Optional on-column DNase treatment for DNA-free RNA
Results
- Detectable and quantifiable RNA was successfully recovered from as few as 100-500 cells cultured in a 3D environment.
- At practical seeding densities of 2,000-10,000 cells, RNA yields ranged from 3-18 ng/µL, providing sufficient material for a wide range of downstream applications, including RT-qPCR, miRNA profiling, and RNA sequencing (RNA-seq).
Why Researchers Love This Combo
- Corning® ULA & Matrigel® – Industry-leading 3D culture platforms
- Promega ReliaPrep™ – Fast, column-based RNA isolation with no phenol/chloroform
- Validated together for 3D samples – save time and get better data
Ready to upgrade your 3D RNA workflow? As an authorized distributor for Promega and Corning in Hong Kong, Bio-Gene is fully committed to supporting your research without interruption. Please contact us today for the full Application Note AN296 and pricing on the ReliaPrep™ RNA Miniprep System + Corning® 3D Cultureware.
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【技術焦點】3D 細胞培養 Total RNA 分離：Promega ReliaPrep™ miRNA系統 與 Corning® ULA / Matrigel®基質
3D 細胞培養 (spheroid 與 organoid culture) 已成為藥物開發、癌症研究與組織工程的標準平台。但從微球體或 Corning Matrigel® 基質中提取高品質 total RNA（含 miRNA）曾經相當棘手。
現在，Promega ReliaPrep™ miRNA Miniprep System 搭配 Corning® Ultra-Low Attachment (ULA) 培養板或 Matrigel® 基質，提供一套完整、經過驗證的 3D RNA 分離方案。
(註：Reliaprep RNA Cell Miniprep及 Reliaprep RNA Tissue Miniprep System將合併為單一統一的 ReliaPrep™ RNA Miniprep System。)
精華步驟
1.在 Corning ULA 或 Matrigel® 中培養細胞
2.使用 LBA + TG 緩衝液溫和勻漿
3.簡單離心柱純化流程（詳見 TM469）
4.可選柱上 DNase 處理，獲得無 DNA 污染的 RNA
結果
- 在 3D 環境下培養，即使僅 100–500 個細胞，仍可成功回收可檢測且可定量之 RNA。
- 在實際接種密度 2,000–10,000 個細胞的條件下，RNA 產量可達 3–18 ng/µL，足以供應多種下游應用，包括 RT-qPCR、miRNA 分析及 RNA 定序（RNA-seq）。
無論您研究的是 spheroid、organoid 還是 3D 侵襲模型，這組合都能讓您快速獲得高品質 RNA，適用於 RT-qPCR、RNA-seq 或 miRNA 分析。
為何研究人員愛用此組合？
- Corning® ULA 與 Matrigel® – 業界領先 3D 培養平台
- Promega ReliaPrep™ – 快速、無酚氯仿的 RNA 分離技術
- 兩者已共同驗證，省時又可靠
想升級您的 3D RNA 工作流程？作為 Promega 與 Corning 香港授權代理商，Bio-Gene 致力於為您的 RNA 研究提供最穩定的支援。立即聯絡我們索取完整 Application Note AN296 及 ReliaPrep™ + Corning® 3D 培養耗材優惠組合！
