Specification
| 632180 |
Lenti-X™ 293T Cell Line, 1 mL |
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| 631275 |
Lenti-X™ Packaging Single Shots (VSV-G), 16 Rxns |
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| 631276 |
Lenti-X™ Packaging Single Shots (VSV-G), 96 Rxns |
| 631278 |
Lenti-X™ Packaging Single Shots (Ecotropic), 16 Rxns |
| 631282 |
Lenti-X™ Packaging Single Shots (96-well, VSV-G), 4 x 96-well plates |
Overview
High-titer, one-step lentivirus packaging systems
Lenti-X packaging single shots are fourth-generation lentiviral packaging systems utilizing an extremely simple, consistent one-step method for producing high-titer lentivirus. Typically, titers of 107–108 IFU per ml can be expected for the VSV-G version. No additional transfection reagent is needed. Lenti-X packaging single shots consist of optimized ratios of Xfect Transfection Reagent and Lenti-X packaging plasmids—lyophilized and pre-aliquoted in single tubes or 96-well plates. High titer virus is produced by simply reconstituting this mixture with your lentiviral vector of choice in sterile water and adding it to 293T cells, e.g., Lenti-X 293T Cell Line (Cat. # 632180).
VSV-G
Provide the highest titers (107–108 IFU per ml), give the broadest tropism, and are provided in single-tube and 96-well formats, respectively.
Ecotropic
Restrict tropism to cells of mouse and rat origin.
Integrase Deficient
Produce VSV-G pseudotyped lentivirus that has a significantly reduced ability to integrate into the host cell genome.
Envelope-Free
Enable the flexibility to produce lentivirus pseudotyped with an envelope protein of choice.
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